期刊
ACS APPLIED MATERIALS & INTERFACES
卷 14, 期 2, 页码 2501-2509出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c17859
关键词
COVID-19; serology; antibody; column agglutination test; polystyrene microbeads; spike protein; clinical samples; diagnostics
资金
- Centre to Impact Antimicrobial Resistance at Monash University
This article describes a rapid diagnostic platform based on column agglutination test for detecting antibodies against SARS-CoV-2. The method is simple, scalable, and can be easily integrated with established laboratory frameworks.
Rapid serology platforms are essential in disease pandemics for a variety of applications, including epidemiological surveillance, contact tracing, vaccination monitoring, and primary diagnosis in resource-limited areas. Laboratory-based enzymelinked immunosorbent assay (ELISA) platforms are inherently multistep processes that require trained personnel and are of relatively limited throughput. As an alternative, agglutination-based systems have been developed; however, they rely on donor red blood cells and are not yet available for high-throughput screening. Column agglutination tests are a mainstay of pretransfusion blood typing and can be performed at a range of scales, ranging from manual through to fully automated testing. Here, we describe a column agglutination test using colored microbeads coated with recombinant SARS-CoV-2 spike protein that agglutinates when incubated with serum samples collected from patients recently infected with SARS-CoV-2. After confirming specific agglutination, we optimized centrifugal force and time to distinguish samples from uninfected vs SARS-CoV-2-infected individuals and then showed concordant results against ELISA for 22 clinical samples, and also a set of serial bleeds from one donor at days 6-10 postinfection. Our study demonstrates the use of a simple, scalable, and rapid diagnostic platform that can be tailored to detect antibodies raised against SARS-CoV-2 and can be easily integrated with established laboratory frameworks worldwide.
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