4.8 Article

Highly Stable Buffer-Based Zinc Oxide/Reduced Graphene Oxide Nanosurface Chemistry for Rapid Immunosensing of SARS-CoV-2 Antigens

期刊

ACS APPLIED MATERIALS & INTERFACES
卷 14, 期 8, 页码 10844-10855

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c24475

关键词

SARS-CoV-2; antigen testing; nucleocapsid proteins; zinc oxide; reduced graphene oxide; electrochemical immunosensing

资金

  1. Canadian Institutes of Health Research (CIHR)
  2. Natural Sciences and Engineering Research of Canada (NSERC)
  3. Canada Research Chair
  4. CMC Microsystems, Canada
  5. University of Calgary

向作者/读者索取更多资源

This study presents a stable buffer-based zinc oxide/reduced graphene oxide nanocomposite coated on carbon screen-printed electrodes for the electrochemical immuno-biosensing of SARS-CoV-2 protein antigens. The biosensor shows a low limit of detection, stable sensor nanocoating, high sensitivity, and the ability to rapidly discriminate positive and negative clinical samples.
The widespread and long-lasting effect of the COVID-19 pandemic has called attention to the significance of technological advances in the rapid diagnosis of SARS-CoV-2 virus. This study reports the use of a highly stable buffer-based zinc oxide/reduced graphene oxide (bbZnO/rGO) nanocomposite coated on carbon screen-printed electrodes for electrochemical immuno-biosensing of SARS-CoV-2 nuelocapsid (N-) protein antigens in spiked and clinical samples. The incorporation of a salt-based (ionic) matrix for uniform dispersion of the nanomixture eliminates multistep nanomaterial synthesis on the surface of the electrode and enables a stable single-step sensor nanocoating. The immuno-biosensor provides a limit of detection of 21 fg/mL over a linear range of 1-10 000 pg/mL and exhibits a sensitivity of 32.07 ohms.mL/pg.mm(2) for detection of N-protein in spiked samples. The N-protein biosensor is successful in discriminating positive and negative clinical samples within 15 min, demonstrating its proof of concept used as a COVID-19 rapid antigen test.

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