期刊
HUMAN PATHOLOGY
卷 53, 期 -, 页码 121-129出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.humpath.2015.11.013
关键词
Esophageal squamous cell carcinoma; PIK3CA; Proliferation; Metastasis; Motility; Prognosis
类别
资金
- Natural Science Foundation of China [81160303, 81260359, 81201891, U1303321, 81360357]
- Major Science and Technology Projects of the Xinjiang Uygur Autonomous Region [201430123-1]
- Xinjiang Key Laboratory of Major Diseases [2014Y3, 2012-Y2]
- Xinjiang Medical University Scientific Research Innovation Foundation [201311]
The PIK3CA mutation has been extensively reported in the setting of cancers; however, the clinicopathological significance of PIK3CA expression has rarely been discussed in esophageal squamous cell carcinoma. In the present study, to confirm the significance of PIK3CA expression in association with metastasis and prognosis, which has been somewhat controversial in esophageal squamous cell carcinoma (ESCC), the relationship between clinicopathological features of ESCC and PIK3CA expression was analyzed using immunohistochemistry with a tissue microarray. Meanwhile, as additional verification and an ethnic control, another independent small cohort of Kazakh ESCC were analyzed by immunohistochemistry. To investigate the pilot role of PIK3CA in ESCC cells, ESCC cell lines ECa109 and EC9706 were transiently transfected with specific siRNA against PIK3CA. The silencing effect was detected by Western blot. Cell proliferation was examined using the 3-(4,5-dirnethyl-2-thiazolyl)-2,5diphenyl-2-H-tetrazolium bromide assay; apoptosis and the cell cycles were analyzed by flow cytometry. Furthermore, the migratory and invasive ability were evaluated by wound healing and transwell invasion assay, respectively. Expression of PIK3CA was significantly higher in ESCC than in paired normal controls and was ethnicity independent; no statistically significant difference was observed between PIK3CA expression and sex, age, depth of invasion, tumor differentiation, lymph node metastasis, or prognosis. Proliferation, migration, and invasion were all markedly reduced after knockout of PIK3CA. Moreover, the cell cycle was arrested at the S phase, and the apoptosis rate was significantly increased, suggesting that PIK3CA plays a key role in promoting the proliferation and motility of ESCC cells. (C) 2016 Elsevier Inc. All rights reserved.
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