期刊
HOLZFORSCHUNG
卷 70, 期 7, 页码 641-652出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/hf-2015-0120
关键词
heartwood; laser microdissection (LMD); lignification; ray parenchyma cell; sapwood; thioacidolysis; time-of-flight secondary ion mass spectrometry (TOF-SIMS)
资金
- Japan Society for the Promotion of Science [25252032, 25114508, 15H01230]
- Grants-in-Aid for Scientific Research [15K07510, 15H01230, 25114508, 25252032] Funding Source: KAKEN
Lignification of ray parenchyma cells (RPCs) in the sapwood (sW), transition zone (TZ), and heartwood (hW) of Phellodendron amurense Rupr. has been investigated by time-of-flight secondary ion mass spectrometry-(TOF-SIMS) and thioacidolysis. The results of TOF-SIMS indicate that the relative ion intensity of lignin in RPCs increased from sW to hW, while there was almost no difference in the case of axial wood fibers. The ratio of syringyl (S) to guaiacyl (G) lignin units (S/G value) in RPCs was higher than that in wood fibers. Samples containing more RPCs were prepared by laser microdissection (LMD) and analyzed by thioacidolysis, including also the subsequent Raney nickel desulfurization, and the resulting monomers and dimers were quantitatively analyzed by GC/MS. The monomer analysis shows that lignin content and S/G values in RPCs rich samples increased from sW towards hW. The dimer analysis reveals that the S-S dimers are dominant in all types of samples. The results of TOF-SIMS and thioacidolysis of LMD samples are interpreted that the lignification of RPCs progresses from the sW towards the hW and that the chemical structure of lignin in RPCs is different from that in axial elements.
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