期刊
GYNECOLOGIC ONCOLOGY
卷 141, 期 1, 页码 166-174出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygyno.2016.02.006
关键词
miR-183; MMP-9; Metastasis; Invasion; Cervical cancer
资金
- National Natural Science Foundation of China [81301763, 81572849]
- Henan Provincial Key Scientific and Technological Projects [142102310473]
Objective. MicroRNAs have been reported to play an important role in the invasion and metastasis of cervical cancer. miR-183 was found to inhibit or promote the invasion and metastasis of multiple solid tumors. However, the roles of miR-183 in cervical cancer are unclear. Methods. In this study, miR-183 expression levels were measured in 53 cervical cancer and 13 normal cervical tissues by qRT-PCR. The effects of forced expression of miR-183 on cervical cancer cells invasion and metastasis were investigated using Transwell uncoated or coated with growth factor-reduced Matrigel for migration or invasion assays, respectively. Results. We found that miR-183 expression levels were significantly down-regulated in cervical cancer tissues compared with normal tissues (0.15 +/- 0.011 to 0.86 +/- 0.049). Ectopic expression of miR-183 resulted in the suppression of invasion and migration of cervical cancer cell lines, siha and Hela cells (p < 0.0001). Bioinformatics analysis revealed that MMP-9 was the potential target of miR-183 and it was found that MMP-9 was remarkably up-regulated in cervical cancer. Furthermore, a dual-luciferase reporter assay showed that MMP-9 as a target of miR-183 (p < 0.0001). The invasion and metastasis ability of siha and Hela was suppressed when MMP-9 was down-regulated in vitro (p < 0.0001), Conclusions. In conclusion, our study revealed that miR-183 might be a tumor suppressor via inhibiting the invasion and metastasis of cervical cancer cells through targeting MMP-9, indicating that miR-183 may be a novel potential therapeutic target for cervical cancer. (C) 2016 Elsevier Inc. All rights reserved.
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