期刊
ACS APPLIED NANO MATERIALS
卷 4, 期 8, 页码 8282-8291出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.1c01457
关键词
nanozyme; bimetallic nanoparticles; colorimetry; cholesterol; enzymatic assay
资金
- Basic Science Research Program through the National Research Foundation (NRF) of Korea [2018R1A6A1A03024231, 2021R1A2C1003566]
- National Research Foundation of Korea [2021R1A2C1003566] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Nanostructured artificial enzymes, specifically citric acid-functionalized rhodium-platinum nanoparticles, exhibit enhanced peroxidase-like activity and can be used for sensitive cholesterol detection.
Nanostructured artificial enzymes, also called nanozymes, are considered effective substitutes for natural enzymes. In this study, citric acid-functionalized rhodium-platinum nanoparticles (CA-RhPt NPs) were synthesized by a simple one-pot synthetic method. Transmission electron microscopy images showed the uniform structure of the CA-RhPt NPs, with an average diameter of 3.5 +/- 0.5 nm, and X-ray photoelectron spectroscopy and zeta potential measurements demonstrated the presence of citric acid in the CA-RhPt NPs. The CA-RhPt NPs exhibited enhanced peroxidase-like activity that catalyzed the reaction between 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) due to the synergistic effects of Rh and Pt. The catalytic mechanism of CA-RhPt NPs was found to decompose H2O2 to a hydroxyl radical (center dot OH), and then, the center dot OH oxidizes the TMB, generating a blue product. The excellent peroxidase-like CA-RhPt NPs were then applied to the colorimetric determination of cholesterol. The CA-RhPt NP-based colorimetry showed sensitive cholesterol detection in the range from 0.08 to 5.0 mM, with a detection limit of 25.7 mu M. The recoveries of cholesterol concentrations from the spiked human serum samples were 96.8% and 106%, which were comparable to those from the commercialized cholesterol detection kit. These results demonstrate the possibility of CA-RhPt NPs as alternatives for natural enzymes and applicability for sensitive cholesterol detection.
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