Mycorrhizal Fungal Diversity and Its Relationship with Soil Properties in Camellia oleifera

Arbuscular mycorrhizal fungi (AMF) are well known for their important physiological implications on diversified host plants, while the information on AMF diversity and its relationship with soil properties of Camellia oleifera is yet not fully understood. In the proposed study, high-throughput sequencing of small subunit ribosomal RNA was performed to analyze the AMF diversity of the rhizosphere and endosphere of 20-year-old C. oleifera Xianglin in the field at Wuhan (China) and their relationship with soil physico-chemical properties. As high as 30.73-41.68% of the roots of C. oleifera were colonized by indigenous AMF with a spore density of 66-111 spores/10 g soil. The surface soil (0-20 cm) showed significantly higher root fungal colonization, spore density, soil hyphal length, and easily extractable glomalin-related soil protein content than the sub-surface soil (20-40 cm). Soil pH value, available K, and NO3--N content affected the root and soil mycorrhizal development, whilst soil pH proved to be the most influential soil property governing their variability. A total of 467 OTUs associated with AMF were detected from the endosphere and rhizosphere, representing 10 genera and 138 species, of which 295 OTUs and 9 genera were jointly observed. The genus Glomus displayed maximum relative abundance (>86%) in both endosphere and rhizosphere. Scutellospora was detected in the endosphere, but absent in the rhizosphere. The endosphere recorded a relatively higher number of OTUs and alpha diversity indices (Shannon, Simpson, and PD index) of AMF than rhizosphere. Our study, hence, revealed that C. oleifera in fields was mainly colonized by Glomus, coupled with comparatively greater AMF diversity in the endosphere than in the rhizosphere, governed predominantly by soil pH, NO3--N content, and available K content.

Automated summary

Arbuscular mycorrhizal fungi (AMF) play a crucial role in the physiological implications of diversified host plants, with a study revealing that Camellia oleifera in fields is mainly colonized by Glomus. Higher AMF diversity was observed in the endosphere compared to the rhizosphere, influenced predominantly by soil pH, NO3--N content, and available K content.