期刊
PHOTONICS
卷 8, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/photonics8090383
关键词
phase modulation; lattice light sheet microscopy; digital holography; fluorescence microscopy
类别
资金
- NIH [RO1 NS111749, R21 DC017292]
- National Institutes of Health [123456]
- National Science Foundation [456789]
Stimuli and cellular processes can lead to cell surface deformations, which may be difficult to image using traditional methods. However, the IHLLS-2L technique allows for quantifying cell membrane deformation in neurons without the need for actuation of a sample stage or detection microscope objective.
Stimuli to excitable cells and various cellular processes can cause cell surface deformations; for example, when excitable cell membrane potentials are altered during action potentials. However, these cellular changes may be at or below the diffraction limit (in dendrites the structures measured are as small as 1 mu m), and imaging by traditional methods is challenging. Using dual lenses incoherent holography lattice light-sheet (IHLLS-2L) detection with holographic phase imaging of selective fluorescent markers, we can extract the full-field cellular morphology or structural changes of the object's phase in response to external stimulus. This approach will open many new possibilities in imaging neuronal activity and, overall, in light sheet imaging. In this paper, we present IHLLS-2L as a well-suited technique for quantifying cell membrane deformation in neurons without the actuation of a sample stage or detection microscope objective.
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