期刊
LIFE-BASEL
卷 11, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/life11060493
关键词
bacteria; biodiversity; landfill; lignocellulose biomass; lignocellulolytic enzyme; lignocellulolytic bacteria; metagenomics
资金
- Universiti Sains Malaysia RUI grant [1001/PTEKIND/8011044]
Omics technology was used to analyze the microbial community in a landfill, revealing high bacterial diversity and significant influence of temperature and moisture content. PICRUSt analysis provided insights into the community's metabolic functions, particularly focusing on lignocellulolytic enzymes. These results contribute to understanding the dominant organisms in landfills and the enzymes involved in lignocellulose breakdown.
Omics have given rise to research on sparsely studied microbial communities such as the landfill, lignocellulolytic microorganisms and enzymes. The bacterial diversity of Municipal Solid Waste sediments was determined using the illumina MiSeq system after DNA extraction and Polymerase chain reactions. Data analysis was used to determine the community's richness, diversity, and correlation with environmental factors. Physicochemical studies revealed sites with mesophilic and thermophilic temperature ranges and a mixture of acidic and alkaline pH values. Temperature and moisture content showed the highest correlation with the bacteria community. The bacterial analysis of the community DNA revealed 357,030 effective sequences and 1891 operational taxonomic units (OTUs) assigned. Forty phyla were found, with the dominant phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidota, while Aerococcus, Stenotrophomonas, and Sporosarcina were the dominant species. PICRUSt provided insight on community's metabolic function, which was narrowed down to search for lignocellulolytic enzymes' function. Cellulase, xylanase, esterase, and peroxidase were gene functions inferred from the data. This article reports on the first phylogenetic analysis of the Pulau Burung landfill bacterial community. These results will help to improve the understanding of organisms dominant in the landfill and the corresponding enzymes that contribute to lignocellulose breakdown.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据