Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus

Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry. Thus, it is crucial to establish a rapid and efficient detection method against PPV infection. In the present work, we developed a recombinase-aided amplification (RAA) assay, coupled with a lateral flow dipstick (LFD), to achieve an amplification of PPV DNA at 37 degrees C within 15 min. The detection limits of PPV RAA-LFD assay were 10(2) copies/mu L recombinant plasmid pMD19-T-VP1, 6.38 x 10(-7) ng/mu L PPV DNA, and 10(-1) TCID50/mL virus, respectively. This method was highly specific for PPV detection with no cross-reactivity for other swine pathogens. In contrast to polymerase chain reaction (PCR), the PPV RAA-LFD assay is more sensitive and cost-saving. Hence, the established PPV RAA-LFD assay provided an alternative for PPV detection, especially in resource-limited regions.

Automated summary

Porcine parvovirus (PPV) infection is a major concern in the swine industry globally, and it is crucial to establish a rapid and efficient detection method. A new recombinase-aided amplification (RAA) assay was developed in this study, which showed high specificity for PPV detection, increased sensitivity compared to PCR, and cost-saving benefits.