期刊
CHEMOSENSORS
卷 9, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/chemosensors9070176
关键词
polydiacetylene; preterm birth; steroid hormone detection
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIP: Ministry of Science, ICT & Future Planning) [NRF2018R1D1A1B07050070]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI19C0185]
- Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program [1711134038]
- BK21 FOUR project through the NRF - Ministry of Education, Korea [F21YY8109033]
A sensing platform based on PDA was developed to detect testosterone as a potential biomarker of preterm birth. By conjugating anti-T antibodies to PDA assemblies and using a mixture of acetonitrile and distilled water as a solvent, the platform showed over 3.4 times higher sensitivity for detecting T compared to other sex steroid hormones studied.
We have developed a polydiacetylene (PDA)-based sensing platform to detect testosterone (T) as a potential biomarker of preterm birth. The insolubility of the steroid hormone in water, where PDA assemblies are dispersed, poses a major issue, since they can hardly interact with each other. To overcome this challenge, acetonitrile was used as a suitable solvent. In addition, to minimize false signals of PDA assemblies caused by the solvent, a mixture of acetonitrile and distilled water was selected. To prove a concept of PDA-based sensing platform for targeting T hormone, we conjugated anti-T antibodies to surface of PDA assemblies to induce selective binding between T and anti-T antibodies. The fluorescence sensory signaling of the PDA-anti-T antibody conjugate was selectively generated for T, over 3.4 times higher sensitivity of the signaling compared to that from other sex steroid hormones studied (beta-estradiol and progesterone).
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