期刊
BIOSENSORS-BASEL
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/bios11090320
关键词
microfluidics; traumatic brain injury; multiplex; immunoassay; biomarkers
资金
- Joyce and Don Massey Family Foundation through the Michigan Center for Integrative Research in Critical Care (MCIRCC) at the University of Michigan
- Microfluidics in Biomedical Sciences Training Program (MBSTP) [T32 EB005582]
- Scholars Awards grant program of Precision Health at the University of Michigan
The study introduced a novel variable height microfluidic device for multiplexing multiple TBI biomarkers simultaneously, which can passively multiplex bead-based immunoassays by trapping beads based on their diameter matching the channel height.
Traumatic brain injury (TBI) is a leading cause of global morbidity and mortality, partially due to the lack of sensitive diagnostic methods and efficacious therapies. Panels of protein biomarkers have been proposed as a way of diagnosing and monitoring TBI. To measure multiple TBI biomarkers simultaneously, we present a variable height microfluidic device consisting of a single channel that varies in height between the inlet and outlet and can passively multiplex bead-based immunoassays by trapping assay beads at the point where their diameter matches the channel height. We developed bead-based quantum dot-linked immunosorbent assays (QLISAs) for interleukin-6 (IL-6), glial fibrillary acidic protein (GFAP), and interleukin-8 (IL-8) using Dynabeads M-450, M-270, and MyOne, respectively. The IL-6 and GFAP QLISAs were successfully multiplexed using a variable height channel that ranged in height from similar to 7.6 mu m at the inlet to similar to 2.1 mu m at the outlet. The IL-6, GFAP, and IL-8 QLISAs were also multiplexed using a channel that ranged in height from similar to 6.3 mu m at the inlet to similar to 0.9 mu m at the outlet. Our system can keep pace with TBI biomarker discovery and validation, as additional protein biomarkers can be multiplexed simply by adding in antibody-conjugated beads of different diameters.
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