期刊
CELL DEATH DISCOVERY
卷 7, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41420-021-00644-4
关键词
-
类别
资金
- Qing Lan Project of Jiangsu Province
- National Natural Science Foundation of China [31772568]
The discovered miR-2337 plays a significant role in the fertility of sows by enhancing TGF-beta 1 promoter activity and altering histone modifications, controlling TGF-beta 1-mediated signaling pathway activity and GC apoptosis.
Transforming growth factor-beta 1 (TGF-beta 1) is essential for ovarian function and female fertility in mammals. Herein, we identified three completely linked variants, including two known variants referred to as c.1583A>G and c.1587A>G and the novel variant c.2074A>C in the porcine TGF-beta 1 3 ' -UTR. An important role of these variants in Yorkshire sow fertility was revealed. Variants c.1583A>G and c.1587A>G were located at the miRNA response element (MRE) of miR-2337 and affected miR-2337 regulation of TGF-beta 1 3 ' -UTR activity. Interestingly, miR-2337 induces, not reduces the transcription and production of TGF-beta 1 in granulosa cells (GCs). Mechanistically, miR-2337 enhances TGF-beta 1 promoter activity via the MRE motif in the core promoter region and alters histone modifications, including H3K4me2, H3K4me3, H3K9me2, and H3K9ac. In addition, miR-2337 controls TGF-beta 1-mediated activity of the TGF-beta signaling pathway and GC apoptosis. Taken together, our findings identify miR-2337 as an endogenous small activating RNA (saRNA) of TGF-beta 1 in GCs, while miR-2337 is identified as a small activator of the TGF-beta signaling pathway which is expected to be a new target for rescuing GC apoptosis and treating low fertility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据