期刊
COMMUNICATIONS BIOLOGY
卷 4, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s42003-021-02559-3
关键词
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资金
- Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), France Genomique [ANR-10-INBS-09-08]
- Center de cooperation Internationale en Recherche Agronomique pour le Developpement (CIRAD)
- Agropolis Fondation [1504-006, ANR-10-LABX-0001-01]
- ERDF project 'Plants as a tool for sustainable global development' [CZ.02.1.01/0.0/0.0/16_019/0000827]
- Genoscope
A chromosome-scale assembly of a banana genome (Musa acuminata) was reported using Oxford Nanopore long-reads, with five out of the eleven chromosomes entirely reconstructed in a single contig from telomere to telomere, revealing the content of complex regions like centromeres or clusters of paralogous genes for the first time.
Belser, Baurens et al. report a chromosome-scale assembly of a banana genome (Musa acuminata) with five out of eleven chromosomes entirely reconstructed in a single contig from telomere to telomere. This work sheds light on the content of complex regions like centromeres or clusters of paralogous genes in the banana genome. Long-read technologies hold the promise to obtain more complete genome assemblies and to make them easier. Coupled with long-range technologies, they can reveal the architecture of complex regions, like centromeres or rDNA clusters. These technologies also make it possible to know the complete organization of chromosomes, which remained complicated before even when using genetic maps. However, generating a gapless and telomere-to-telomere assembly is still not trivial, and requires a combination of several technologies and the choice of suitable software. Here, we report a chromosome-scale assembly of a banana genome (Musa acuminata) generated using Oxford Nanopore long-reads. We generated a genome coverage of 177X from a single PromethION flowcell with near 17X with reads longer than 75 kbp. From the 11 chromosomes, 5 were entirely reconstructed in a single contig from telomere to telomere, revealing for the first time the content of complex regions like centromeres or clusters of paralogous genes.
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