4.7 Article

Auto-inhibitory intramolecular S5/S6 interaction in the TRPV6 channel regulates breast cancer cell migration and invasion

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COMMUNICATIONS BIOLOGY
卷 4, 期 1, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/s42003-021-02521-3

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  1. Natural Sciences and Engineering Research Council of Canada (NSERC)
  2. Kidney Foundation of Canada
  3. National Natural Science Foundation of China [31871176, 32070726]

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TRPV6, a calcium-selective channel, plays a crucial role in calcium homeostasis and its specific mutation may promote the progression of breast cancer cells.
TRPV6, a Ca-selective channel, is abundantly expressed in the placenta, intestine, kidney and bone marrow. TRPV6 is vital to Ca homeostasis and its defective expression or function is linked to transient neonatal hyperparathyroidism, Lowe syndrome/Dent disease, renal stone, osteoporosis and cancers. The fact that the molecular mechanism underlying the function and regulation of TRPV6 is still not well understood hampers, in particular, the understanding of how TRPV6 contributes to breast cancer development. By electrophysiology and Ca imaging in Xenopus oocytes and cancer cells, molecular biology and numerical simulation, here we reveal an intramolecular S5/S6 helix interaction in TRPV6 that is functionally autoinhibitory and is mediated by the R532:D620 bonding. Predicted pathogenic mutation R532Q within S5 disrupts the S5/S6 interaction leading to gain-of-function of the channel, which promotes breast cancer cell progression through strengthening of the TRPV6/PI3K interaction, activation of a PI3K/Akt/GSK-3 beta cascade, and up-regulation of epithelial-mesenchymal transition and anti-apoptosis. Cai et al discover an auto-inhibitory interaction between the S5 and S6 helices in the calcium-selective channel TRPV6. Molecular dynamics simulations predict that pathogenic mutation R532Q in S5 results in channel gain-of-function, which is confirmed in breast cancer migration, invasion and apoptosis assays.

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