期刊
PHARMACEUTICALS
卷 14, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/ph14060509
关键词
Pt(II) complex; anticancer activity; apoptosis; DNA fragmentation; p53; caspase-8
资金
- Research Center of the Female Scientific and Medical Colleges, King Saud University
The study showed that the Pt(II)-thiocarbohydrazone complex exhibited significant cytotoxic effects against ovarian and colorectal adenocarcinoma cells, triggering apoptosis through the activation of the p53 and caspase-8 pathways. These findings suggest that the complex could be a promising chemotherapeutic agent for various types of cancer cells.
In this study, a platinum(II) complex ([Pt(H2L)(PPh3)] complex) containing a thiocarbohydrazone as the ligand was tested as an anti-proliferative agent against ovarian adenocarcinoma (Caov-3) and human colorectal adenocarcinoma (HT-29) through MTT assays. Apoptotic markers were tested by the AO/PI double staining assay and DNA fragmentation test. Flow cytometry was conducted to measure cell cycle distribution, while the p53 and caspase-8 pathways were tested via immunofluorescence assay. Results demonstrated that the cytotoxic effect of the Pt(II)-thiocarbohydrazone complexes against Caov-3 and HT-29 cells was highly significant, and this effect triggered the activation of the p53 and caspase-8 pathways. Besides, apoptosis stimulated by the Pt(II)-thiocarbohydrazone complex was associated with cell cycle arrest at the G0/G1 phase. These findings suggest that the target complex inhibited the proliferation of Caov-3 and HT-29 cells, resulting in the arrest of the cell cycle and induction of apoptosis via the stimulation of the p53 and caspase-8 pathways. The present data suggests that the Pt(II)-thiocarbohydrazone complex could also be a promising chemotherapeutic agent for other types of cancer cells.
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