期刊
JOURNAL OF PERSONALIZED MEDICINE
卷 11, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/jpm11060448
关键词
endometrium; endometrial regeneration; wound healing; mesenchymal stromal cell; endometrial pericyte
资金
- Jane and Dan Olsson Foundation
- Swedish research council [2017-00932]
- Karolinska Institutet (KID) [2-3591/2014]
By utilizing single-cell RNA sequencing, researchers identified multiple stromal populations in the endometrium with potential roles in controlling inflammation and extracellular matrix composition. These findings contribute to a better understanding of healthy regeneration mechanisms and may lead to the identification of novel therapy targets for gynecological disorders affecting endometrial regeneration and proliferation.
The endometrium undergoes regular regeneration and stromal proliferation as part of the normal menstrual cycle. To better understand cellular interactions driving the mechanisms in endometrial regeneration we employed single-cell RNA sequencing. Endometrial biopsies were obtained during the proliferative phase of the menstrual cycle from healthy fertile women and processed to single-cell suspensions which were submitted for sequencing. In addition to known endometrial cell types, bioinformatic analysis revealed multiple stromal populations suggestive of specific stromal niches with the ability to control inflammation and extracellular matrix composition. Ten different stromal cells and two pericyte subsets were identified. Applying different R packages (Seurat, SingleR, Velocyto) we established cell cluster diversity and cell lineage/trajectory, while using external data to validate our findings. By understanding healthy regeneration in the described stromal compartments, we aim to identify points of further investigation and possible targets for novel therapy development for benign gynecological disorders affecting endometrial regeneration and proliferation such as endometriosis and Asherman's syndrome.
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