期刊
ISCIENCE
卷 24, 期 9, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2021.103029
关键词
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资金
- BBSRC [BB/R00787X/1]
- MRC [MR/L003791/1]
- Leverhulme Trust [RPG-2019-191]
- Wellcome Trust [105384/Z/14/A]
- Wellcome Trust [105384/Z/14/A] Funding Source: Wellcome Trust
- BBSRC [BB/R00787X/1] Funding Source: UKRI
- MRC [MR/L003791/1] Funding Source: UKRI
The study found that sustained activation of GluK2 subunit-containing kainate receptors leads to AMPAR endocytosis and induces LTD in hippocampal neurons. In acute hippocampal slices, kainate application causes a significant loss of GluA2-containing AMPARs from synapses and long-lasting depression of AMPAR excitatory postsynaptic currents in CA1. These results demonstrate that kainate receptors can bidirectionally regulate synaptic AMPARs and synaptic plasticity via different signaling pathways.
It is well established that long-term depression (LTD) can be initiated by either NMDA or mGluR activation. Here we report that sustained activation of GluK2 subunit-containing kainate receptors (KARs) leads to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) endocytosis and induces LTD of AMPARs (KAR-LTDAMPAR) in hippocampal neurons. The KAR-evoked loss of surface AMPARs is blocked by the ionotropic KAR inhibitor UBP 310 indicating that KAR-LTDAMPAR requires KAR channel activity. Interestingly, however, blockade of PKC or PKA also reduces GluA2 surface expression and occludes the effect of KAR activation. In acute hippocampal slices, kainate application caused a significant loss of GluA2-containing AMPARs from synapses and long-lasting depression of AMPAR excitatory postsynaptic currents in CA1. These data, together with our previously reported KAR-LTPAMPAR, demonstrate that KARs can bidirectionally regulate synaptic AMPARs and synaptic plasticity via different signaling pathways.
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