The Hippo pathway uses different machinery to control cell fate and organ size

The Hippo pathway is a conserved signaling network that regulates organ growth and cell fate. One such cell fate decision is that of R8 photoreceptor cells in the Drosophila eye, where Hippo specifies whether cells sense blue or green light. We show that only a subset of proteins that control organ growth via the Hippo pathway also regulate R8 cell fate choice, including the STRIPAK complex, Tao, Pez, and 14-3-3 proteins. Furthermore, key Hippo pathway proteinswere primarily cytoplasmic in R8 cells rather than localized to specific membrane domains, as in cells of growing epithelial organs. Additionally, Warts was the only Hippo pathway protein to be differentially expressed between R8 subtypes, while central Hippo pathway proteins were expressed at dramatically lower levels in adult and pupal eyes than in growing larval eyes. Therefore, we reveal several important differences in Hippo signaling in the contexts of organ growth and cell fate.

Automated summary

The Hippo pathway plays a critical role in regulating organ growth and cell fate, specifically in the context of R8 photoreceptor cells. The study reveals important differences in the expression and localization of key proteins in the Hippo pathway between R8 cells and growing epithelial organs, shedding light on the complex signaling network involved in organ growth and cell fate decision.