期刊
BLOOD ADVANCES
卷 5, 期 14, 页码 2817-2828出版社
ELSEVIER
DOI: 10.1182/bloodadvances.2020003795
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类别
资金
- Fondazione Associazione Italiana per la Ricerca sul Cancro, AIRC (FIRC) 5 per 1000 Molecular Clinical Oncology Special Program [9965]
- AIRC/FIRC
- Fondazione Associazione Italiana per la Ricerca sul Cancro, AIRC (FIRC) 5 per 1000 2019 [22737]
The presence of CD4(+) T cells is essential for the development of chronic lymphocytic leukemia (CLL), while CD8(+) T cells have an antitumor effect in restraining CLL progression. Antigen specificity of CD4(+) T cells has marginal impact on CLL development.
Chronic lymphocytic leukemia (CLL) is caused by the progressive accumulation of mature CD5(+) B cells in secondary lymphoid organs. In vitro data suggest that CD4(+) T lymphocytes also sustain survival and proliferation of CLL clones through CD40L/CD40 interactions. In vivo data in animal models are conflicting. To clarify this clinically relevant biological issue, we generated genetically modified E mu-TCL1 mice lacking CD4(+) T cells (TCL1(+/+)AB0), CD40 (TCL1(+/+)CD40(-/-)), or CD8(+) T cells (TCL1(+/+)TAP(-/-)), and we monitored the appearance and progression of a disease that mimics aggressive human CLL by flow cytometry and immunohistochemical analyses. Findings were confirmed by adoptive transfer of leukemic cells into mice lacking CD4(+) T cells or CD40L or mice treated with antibodies depleting CD4 T cells or blocking CD40L/CD40 interactions. CLL clones did not proliferate in mice lacking or depleted of CD4(+) T cells, thus confirming that CD4(+) T cells are essential for CLL development. By contrast, CD8(+) T cells exerted an antitumor activity, as indicated by the accelerated disease progression in TCL1(+/+)TAP(-/-) mice. Antigen specificity of CD4(+) T cells was marginal for CLL development, because CLL clones efficiently proliferated in transgenic mice whose CD4 T cells had a T-cell receptor with CLL-unrelated specificities. Leukemic clones also proliferated when transferred into wild-type mice treated with monoclonal antibodies blocking CD40 or into CD40L(-/-) mice, and TCL1(+/+)CD40(-/-) mice developed frank CLL. Our data demonstrate that CD8(+) T cells restrain CLL progression, whereas CD4(+) T cells support the growth of leukemic clones in TCL1 mice through CD40-independent and apparently noncognate mechanisms.
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