期刊
JOURNAL OF FUNGI
卷 7, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/jof7070529
关键词
Histoplasma capsulatum; bat host; NAm 3 clade; new lineage; phylogenetic reconstruction; concatenated sequence-types network
资金
- UNAM-Mexico (PAPIIT-DGAPA/UNAM-MX) [IN213515]
The study reveals at least 11 phylogenetic species of Histoplasma capsulatum, with a clade (BAC1) containing isolates from infected bats in Mexico. Additionally, isolates from migratory bats and specific bat species support the NAm 3 clade, replacing the previously reported BAC1 clade. Concatenated sequence analyses validate these findings, suggesting a relationship between NAm 3 and the North American class 2 clade.
Histoplasma capsulatum is a dimorphic fungus associated with respiratory and systemic infections in mammalian hosts that have inhaled infective mycelial propagules. A phylogenetic reconstruction of this pathogen, using partial sequences of arf, H-anti, ole1, and tub1 protein-coding genes, proposed that H. capsulatum has at least 11 phylogenetic species, highlighting a clade (BAC1) comprising three H. capsulatum isolates from infected bats captured in Mexico. Here, relationships for each individual locus and the concatenated coding regions of these genes were inferred using parsimony, maximum likelihood, and Bayesian inference methods. Coalescent-based analyses, a concatenated sequence-types (CSTs) network, and nucleotide diversities were also evaluated. The results suggest that six H. capsulatum isolates from the migratory bat Tadarida brasiliensis together with one isolate from a Mormoops megalophylla bat support a NAm 3 clade, replacing the formerly reported BAC1 clade. In addition, three H. capsulatum isolates from T. brasiliensis were classified as lineages. The concatenated sequence analyses and the CSTs network validate these findings, suggesting that NAm 3 is related to the North American class 2 clade and that both clades could share a recent common ancestor. Our results provide original information on the geographic distribution, genetic diversity, and host specificity of H. capsulatum.
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