4.6 Article

Acute Exposure to Bisphenol A Causes Oxidative Stress Induction with Mitochondrial Origin in Saccharomyces cerevisiae Cells

期刊

JOURNAL OF FUNGI
卷 7, 期 7, 页码 -

出版社

MDPI
DOI: 10.3390/jof7070543

关键词

bisphenol A; nuclear genome integrity maintenance; ROS production; DNA oxidative damage; protein carbonyl content; mitochondrial involvement

资金

  1. Slovak Research and Development Agency [APVV14-0783]
  2. [VEGA 2/0146/20]
  3. [VEGA 1/0460/21]

向作者/读者索取更多资源

BPA, a major component in common plastic products, acts as an endocrine disrupting compound with adverse physiological effects. High concentrations of BPA affect cell survival and increase intracellular oxidation, primarily in the mitochondrion, but acute exposure does not cause significant oxidative damage to DNA or proteins.
Bisphenol A (BPA) is a major component of the most commonly used plastic products, such as disposable plastics, Tetra Paks, cans, sport protective equipment, or medical devices. Due to the accumulation of excessive amounts of plastic waste and the subsequent release of BPA into the environment, BPA is classified as a pollutant that is undesirable in the environment. To date, the most interesting finding is the ability of BPA to act as an endocrine disrupting compound due to its binding to estrogen receptors (ERs), and adverse physiological effects on living organisms may result from this action. Since evidence of the potential pro-oxidizing effects of BPA has accumulated over the last years, herein, we focus on the detection of oxidative stress and its origin following BPA exposure using pulsed-field gel electrophoresis, flow cytometry, fluorescent microscopy, and Western blot analysis. Saccharomyces cerevisiae cells served as a model system, as these cells lack ERs allowing us to dissect the ER-dependent and -independent effects of BPA. Our data show that high concentrations of BPA affect cell survival and cause increased intracellular oxidation in yeast, which is primarily generated in the mitochondrion. However, an acute BPA exposure does not lead to significant oxidative damage to DNA or proteins.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据