4.5 Article

Novel nanoformulation of disulfiram with bacterially synthesized copper oxide nanoparticles for augmenting anticancer activity: an in vitro study

期刊

CANCER NANOTECHNOLOGY
卷 12, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s12645-021-00097-5

关键词

Disulfiram; Copper oxide nanoparticles; Disulfiram-copper oxide nanoparticles; Cellular uptake; Anticancer

向作者/读者索取更多资源

A novel nanocomplex of DS-CuO NPs was prepared, showing high stability and potent selective anticancer activity against human breast, lung, and liver cancer cells with low inhibitory concentration. This nanocomplex exhibited better anticancer effect and cellular uptake, as well as prooxidant effect-mediated apoptosis and anti-metastatic potential. These findings suggest that the novel nanocomplex may improve the therapeutic index of the conventional DS-Cu complex and warrant further investigation for future clinical applications.
Background: Disulfiram (DS), in the presence of copper (Cu), exhibited potent broad anticancer activity. However, its clinical application is limited due to the poor solubility and stability. Hence, a novel nanocombination of DS with bacterially synthesized copper oxide nanoparticles (CuO NPs) was prepared herein to improve the anticancer efficacy of the typical DS-Cu complex. Our design utilized the nanocharacterization and prooxidant effect-mediated anticancer activity of CuO NPs which may lead to enhanced cellular uptake and thus improved anticancer efficacy of this unique nanocomplex. Results: The characterized DS-CuO NPs exhibited high stability in serum and the strongest selective anticancer activity, with the lowest half-maximum inhibitory concentration (IC50 < 15 nM), against human breast, lung and liver cancer cells, by >10-fold, compared to DS-Cu, CuO NPs and Cu. Importantly, DS-CuO NPs revealed better synergistic anticancer effect and higher cellular uptake than DS-Cu. Moreover, this novel nanocomplex showed higher prooxidant effect-mediated apoptosis and anti-metastatic potential. This was accomplished by elevating cellular reactive species content with inhibiting the anti-oxidant defenders (functional marker of cancer stem cells (aldehyde dehydrogenase) and nuclear factor erythroid 2-related factor2), matrix metallopeptidase 9 and NF-kappa B as well as enhancing p53 expression. Conclusion: All of the aforementioned findings verified that this novel nanocomplex was capable of improving the therapeutic index of the conventional DS-Cu complex. The potent selective anticancer activity of this promising nanomedicine merits further investigation, as a separate future study, using animal models as preliminary step before its clinical application.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据