期刊
BIOMEDICINES
卷 9, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines9070732
关键词
bone regeneration; volume-stable collagen matrix; collagen matrix; histology; qBEI
资金
- AUVA (Research funds of the Austrian workers' compensation board)
- Osteology Foundation
In a rat cranial defect, VSCM shows limited and heterogeneous capacity to support intramembranous bone formation, but may potentially allow bone formation via the endochondral route.
Volume-stable collagen matrices (VSCM) are conductive for the connective tissue upon soft tissue augmentation. Considering that collagen has osteoconductive properties, we have investigated the possibility that the VSCM also consolidates with the newly formed bone. To this end, we covered nine rat calvaria circular defects with a VSCM. After four weeks, histology, histomorphometry, quantitative backscattered electron imaging, and microcomputed tomography were performed. We report that the overall pattern of mineralization inside the VSCM was heterogeneous. Histology revealed, apart from the characteristic woven bone formation, areas of round-shaped hypertrophic chondrocyte-like cells surrounded by a mineralized extracellular matrix. Quantitative backscattered electron imaging confirmed the heterogenous mineralization occurring within the VSCM. Histomorphometry found new bone to be 0.7 mm(2) (0.01 min; 2.4 max), similar to the chondrogenic mineralized extracellular matrix with 0.7 mm(2) (0.0 min; 4.2 max). Microcomputed tomography showed the overall mineralized tissue in the defect to be 1.6 mm(3) (min 0.0; max 13.3). These findings suggest that in a rat cranial defect, VSCM has a limited and heterogeneous capacity to support intramembranous bone formation but may allow the formation of bone via the endochondral route.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据