期刊
NANOIMPACT
卷 23, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.impact.2021.100338
关键词
Iron oxide nanoparticles; GSH; GST antioxidant system; Glutathione consumption; Glutathione S-transferase activity; Biomarkers
资金
- National Science Foundation of China [81872651, 92043202, 91943301]
- Ministry of Science and Technology of China (National Key Research and Development Project) [2017YFC1600200]
The research discovered that Fe3O4 nanoparticles at lower concentrations are more toxic to human bronchial epithelial cells, leading to depletion of GSH and decrease in GST activity, causing further lipid peroxidation.
Glutathione (GSH) and glutathione-S-transferases (GSTs) are two frontlines of cellular defense against both acute and chronic toxicity of xenobiotics-induced oxidative stress. The contribution of GSH and GST enzymes to signaling pathways and the regulation of GSH homeostasis play a central role in the detoxification of numerous environmental toxins and impurities. Iron oxide nanoparticles stemmed from traffic exhaust, steel manufacturing, or welding as a potential environmental pollution can lead to adverse respiratory outcomes and aggravate the risk of chronic health conditions via persistent oxidative stress. In this work, two kinds of acute exposure experiments of iron oxide (Fe2O3 and Fe3O4) nanoparticles in cells and in vivo were conducted to evaluate the GSH levels and GST activity. Our current research presented Fe3O4 nanoparticles at lower concentrations (<= 100 mu g/ml) seem to be more toxic to the human bronchial epithelial cells as their consumption of GSH and decrease of GST activity. The catalysis activity of Fe3O4 nanoparticles per se may contribute to the intracellular GSH consumption along with inhibition of glutathione-S-transferase class mu 1 and P (GSTM1 and GSTP1) active site and expression decrease of GSTM1 and GSTP1. Accordingly, the GSH consumption and decrease in GST activity directed to the further lipid peroxidation regarded as an earlier marker for toxicity evaluation of iron oxide nanoparticles, and relevant intervention may be effective for prevention of respiratory exposure induced damage from iron oxide nanoparticles.
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