期刊
BIOACTIVE MATERIALS
卷 6, 期 8, 页码 2250-2260出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.12.029
关键词
Biomimetic mineralization; Antimicrobial; Cationic and amphipathic peptides; Hard tissue cytocompatibility
资金
- National Institute for Dental and Craniofacial Research of the National Institutes of Health [R01DE026117, T90DE0227232]
- National Institutes of Health's National Center for Advancing Translational Sciences [UL1TR002494]
- Fundamental Research Funds for the Central Universities [2042020kf0191]
- National Natural Science Foundation of China [81400506]
- Natural Science Foundation of Guangdong Province [2018B030311040]
- 3 M Science and Technology Fellowship
- NSF through the MRSEC program
The study developed a scaffold with dual function for promoting tissue growth and preventing bacterial infections. The scaffolds were fabricated through intrafibrillar-mineralization of collagen and coating with antimicrobial peptides, demonstrating both antimicrobial properties and cytocompatibility to human cells. The process of scaffold fabrication is versatile and can be used with other biopolymers for controlling mineral load.
Infection in hard tissue regeneration is a clinically-relevant challenge. Development of scaffolds with dual function for promoting bone/dental tissue growth and preventing bacterial infections is a critical need in the field. Here we fabricated hybrid scaffolds by intrafibrillar-mineralization of collagen using a biomimetic process and subsequently coating the scaffold with an antimicrobial designer peptide with cationic and amphipathic properties. The highly hydrophilic mineralized collagen scaffolds provided an ideal substrate to form a dense and stable coating of the antimicrobial peptides. The amount of hydroxyapatite in the mineralized fibers modulated the rheological behavior of the scaffolds with no influence on the amount of recruited peptides and the resulting increase in hydrophobicity. The developed scaffolds were potent by contact killing of Gram-negative Escherichia coli and Gram-positive Streptococcus gordonii as well as cytocompatible to human bone marrow-derived mesenchymal stromal cells. The process of scaffold fabrication is versatile and can be used to control mineral load and/or intrafibrillar-mineralized scaffolds made of other biopolymers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据