期刊
BIOACTIVE MATERIALS
卷 6, 期 7, 页码 1932-1944出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.12.009
关键词
Extracellular matrix; Waterborne polyurethane; Low-temperature deposition manufacturing; Articular cartilage; Tissue engineering
资金
- National Key R&D Program of China [2018YFC1105900]
- National Natural Science Foundation of China [81772319]
- Natural Science Foundation of Beijing Municipality [7204270]
- Beijing JST Research Funding [ZR-201908]
- Innovation Fund for Outstanding Doctoral Candidates of Peking University Health Science Center [71013Y2029]
This study constructed WPU and WPU-ECM scaffolds utilizing decellularized cartilage extracellular matrix and waterborne polyurethane, achieving hierarchical macro-microporous structures. The optimized WPU-ECM scaffold showed improved cell distribution, adhesion, and proliferation, facilitating the production of glycosaminoglycan and collagen as well as the upregulation of cartilage-specific genes. In vivo studies demonstrated successful regeneration of hyaline cartilage with similar histological structure and mechanical performance to normal cartilage using the hierarchical macro-microporous WPU-ECM scaffold.
Tissue engineering provides a promising avenue for treating cartilage defects. However, great challenges remain in the development of structurally and functionally optimized scaffolds for cartilage repair and regeneration. In this study, decellularized cartilage extracellular matrix (ECM) and waterborne polyurethane (WPU) were employed to construct WPU and WPU-ECM scaffolds by water-based 3D printing using low-temperature deposition manufacturing (LDM) system, which combines rapid deposition manufacturing with phase separation techniques. The scaffolds successfully achieved hierarchical macro-microporous structures. After adding ECM, WPU scaffolds were markedly optimized in terms of porosity, hydrophilia and bioactive components. Moreover, the optimized WPU-ECM scaffolds were found to be more suitable for cell distribution, adhesion, and proliferation than the WPU scaffolds. Most importantly, the WPU-ECM scaffold could facilitate the production of glycosaminoglycan (GAG) and collagen and the upregulation of cartilage-specific genes. These results indicated that the WPU-ECM scaffold with hierarchical macro-microporous structures could recreate a favorable microenvironment for cell adhesion, proliferation, differentiation, and ECM production. In vivo studies further revealed that the hierarchical macro-microporous WPU-ECM scaffold combined with the microfracture procedure successfully regenerated hyaline cartilage in a rabbit model. Six months after implantation, the repaired cartilage showed a similar histological structure and mechanical performance to that of normal cartilage. In conclusion, the hierarchical macro-microporous WPU-ECM scaffold may be a promising candidate for cartilage tissue engineering applications in the future.
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