4.6 Article

Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus

期刊

AQUACULTURE REPORTS
卷 20, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.aqrep.2021.100731

关键词

Sex-linked SNP; Competitive allele-specific PCR assay; KASP; Portunus trituberculatus

资金

  1. National Key R&D Program of China [2018YFD0900303]
  2. Natural Science Foundation of Zhejiang Province [LY17C190005]
  3. Ministry of Agriculture of China & China Agriculture Research System [CARS-48]
  4. Scientific Research Foundation of Graduate School of Ningbo University [IF2020150]
  5. CSC Scholarship [201708330421]
  6. K.C. Wong Magana Fund in Ningbo University

向作者/读者索取更多资源

The study validated two sex-linked SNPs from simplified genomic data, providing a rapid test for sex identification in Portunus trituberculatus, and revealed significant genetic evidence from heterogametic genotypes in males supporting an XX/XY sex determination system.
The swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference and enhanced growth performance. However, the lack of a rapid and reliable sex-linked marker panel has hampered the application of sex control and understanding of the sex determination in the species. In this study, two sex-linked SNPs obtained from our simplified genomic data were validated by sequencing and then converted into competitive allele-specific PCR assays. The results showed high consistency between the genetic and phenotypic sex providing therefore a rapid test sex identification. Moreover, heterogametic genotypes in males provided a significant genetic evidence to support an XX/XY sex determination system in the P. trituberculatus.

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