期刊
FRONTIERS IN PHYSICS
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphy.2021.698343
关键词
non-diffracting; Bessel beam; Airy beam; lattice beam; two-photon microscopy; Raman microscopy
资金
- Research Grants Council of the Hong Kong Special Administrative Region, China [HKU 17205321, HKU 17200219, HKU 17209018, E-HKU701/17, HKU C7047-16G, CityU T42-103/16-N]
- National Natural Science Foundation of China (NSFC) [N_HKU712/16]
- Fudan University [JIF2641001]
The propagation of light in a medium involves diffraction, dispersion, and scattering effects. Research on non-diffracting beams, especially in biomedical applications, can provide fast imaging and scattering-resilient imaging, which is particularly important for sparse sample structures.
The light propagation in the medium normally experiences diffraction, dispersion, and scattering. Studying the light propagation is a century-old problem as the photons may attenuate and wander. We start from the fundamental concepts of the non-diffracting beams, and examples of the non-diffracting beams include but are not limited to the Bessel beam, Airy beam, and Mathieu beam. Then, we discuss the biomedical applications of the non-diffracting beams, focusing on linear and nonlinear imaging, e.g., light-sheet fluorescence microscopy and two-photon fluorescence microscopy. The non-diffracting photons may provide scattering resilient imaging and fast speed in the volumetric two-photon fluorescence microscopy. The non-diffracting Bessel beam and the Airy beam have been successfully used in volumetric imaging applications with faster speed since a single 2D scan provides information in the whole volume that adopted 3D scan in traditional scanning microscopy. This is a significant advancement in imaging applications with sparse sample structures, especially in neuron imaging. Moreover, the fine axial resolution is enabled by the self-accelerating Airy beams combined with deep learning algorithms. These additional features to the existing microscopy directly realize a great advantage over the field, especially for recording the ultrafast neuronal activities, including the calcium voltage signal recording. Nonetheless, with the illumination of dual Bessel beams at non-identical orders, the transverse resolution can also be improved by the concept of image subtraction, which would provide clearer images in neuronal imaging.
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