期刊
DIVERSITY-BASEL
卷 13, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/d13080378
关键词
arabinogalactan protein; cell wall; pectin; hemicellulose; transfer cell; wall ingrowth; Physcomitrium patens
资金
- National Science Foundation [NSF 1758497]
- National Institutes of Health [NIH 5R25GM107760-07]
The study investigated the composition of cell wall polymers in Physcomitrium patens transfer cells using immunogold labeling TEM. The findings showed less pectins and AGPs in transfer cell walls compared to hornwort and liverwort, with similar composition on both sides of the placenta in P. patens. The variability in cell wall composition among bryophytes suggests that function and morphology do not necessarily require a common composition.
Following similar studies of cell wall constituents in the placenta of Phaeoceros and Marchantia, we conducted immunogold labeling TEM studies of Physcomitrium patens to determine the composition of cell wall polymers in transfer cells on both sides of the placenta. Sixteen monoclonal antibodies were used to localize cell wall epitopes in the basal walls and wall ingrowths in this moss. In general, placental transfer cell walls of P. patens contained fewer pectins and far fewer arabinogalactan proteins AGPs than those of the hornwort and liverwort. P. patens also lacked the differential labeling that is pronounced between generations in the other bryophytes. In contrast, transfer cell walls on either side of the placenta of P. patens were relatively similar in composition, with slight variation in homogalacturonan HG pectins. Compositional similarities between wall ingrowths and primary cell walls in P. patens suggest that wall ingrowths may simply be extensions of the primary cell wall. Considerable variability in occurrence, abundance, and types of polymers among the three bryophytes and between the two generations suggested that similarity in function and morphology of cell walls does not require a common cell wall composition. We propose that the specific developmental and life history traits of these plants may provide even more important clues in understanding the basis for these differences. This study significantly builds on our knowledge of cell wall composition in bryophytes in general and in transfer cells across plants.
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