期刊
GLYCOCONJUGATE JOURNAL
卷 33, 期 3, 页码 261-272出版社
SPRINGER
DOI: 10.1007/s10719-016-9649-3
关键词
Glycoproteomics; Glycopeptides; Oxonium ions; Mass spectrometry; Collision-induced dissociation; Higher-energy collisional dissociation; Electron capture dissociation; Electron transfer dissociation
资金
- Demensforbundet, WoM Lundgren
- Alzheimer foundation, Sweden
The use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MSn) for the glycoproteomic characterization of glycopeptides is a growing field of research. The N- and O-glycosylated peptides (N- and O-glycopeptides) analyzed typically originate from protease-digested glycoproteins where many of them are expected to be biomedically important. Examples of LC-MS2 and MS3 fragmentation strategies used to pursue glycan structure, peptide identity and attachment-site identification analyses of glycopeptides are described in this review. MS2 spectra, using the CID and HCD fragmentation techniques of a complex biantennary N-glycopeptide and a core 1 O-glycopeptide, representing two examples of commonly studied glycopeptide types, are presented. A few practical tips for accomplishing glycopeptide analysis using reversed-phase LC-MSn shotgun proteomics settings, together with references to the latest glycoproteomic studies, are presented.
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