期刊
DIAGNOSTICS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/diagnostics11081509
关键词
saliva; COVID-19 diagnosis; coronavirus; SARS-CoV-2
资金
- French Defence Innovation Agency-Agence de l'Innovation de defense (AID, CoviDiagMS Project) [2020-COVID19-15]
- French General Armament Directorate-Direction Generale de l'Armement (DGA, MoSIS project) [PDH-2-NRBC-2-B-2113]
The study assessed the concordance level of SARS-CoV-2 detection between paired nasopharyngeal swabs (NPS) and saliva collected with Salivette(R) at two time points, revealing that the low sensitivity of saliva specimens for SARS-CoV-2 detection may be attributed to mouth washing decreasing viral load in the buccal cavity.
Background: A previous study demonstrated the performance of the Salivette (R) (SARST-EDT, Numbrecht, Germany) as a homogeneous saliva collection system to diagnose COVID-19 by RT-qPCR, notably for symptomatic and asymptomatic patients. However, for convalescent patients, the corroboration of molecular detection of SARS-CoV-2 in paired nasopharyngeal swabs (NPS) and saliva samples was unsatisfactory. Objectives: The aim of the present work was to assess the concordance level of SARS-CoV-2 detection between paired sampling of NPSs and saliva collected with Salivette (R) at two time points, with ten days of interval. Results: A total of 319 paired samples from 145 outpatients (OP) and 51 healthcare workers (HW) were collected. Unfortunately, at day ten, 73 individuals were lost to follow-up, explaining some kinetic missing data. Due to significant waiting rates at hospitals, most of the patients ate and/or drank while waiting for their turn. Consequently, mouth washing was systematically proposed prior to saliva collection. None of the HW were diagnosed as SARS-CoV-2 positive using NPS or saliva specimens at both time points (n = 95) by RT-qPCR. The virus was detected in 56.3% (n = 126/224) of the NPS samples from OP, but solely 26.8% (n = 60/224) of the paired saliva specimens. The detection of the internal cellular control, the human RNase P, in more than 98% of the saliva samples, underlined that the low sensitivity of saliva specimens (45.2%) for SARS-CoV-2 detection was not attributed to an improper saliva sample storing or RNA extraction. Conclusions: This work revealed that mouth washing decreased viral load of buccal cavity conducting to impairment of SARS-CoV-2 detection. Viral loads in saliva neo-produced appeared insufficient for molecular detection of SARS-CoV-2. At the time when saliva tests could be a rapid, simple and non-invasive strategy to assess large scale schoolchildren in France, the determination of the performance of saliva collection becomes imperative to standardize procedures.
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