期刊
PLANTS-BASEL
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/plants10071440
关键词
candidate gene association mapping; intron length polymorphism; single nucleotide polymorphism; girth growth; rubber tree
资金
- Rubber Authority of Thailand
- Thammasat University
This study performed candidate gene association mapping for girth growth in rubber trees using ILP markers and identified COBL064_1 marker as the strongest associated with girth growth. Through CCS analysis, a SNP in the 3' UTR showing linkage disequilibrium with COBL064_1 was pinpointed. The study demonstrated that ILP gene-based markers can assist in identifying SNPs in candidate genes associated with girth growth and provide useful markers for improvement in rubber tree breeding programs.
Girth growth is an important factor in both latex and timber production of the rubber tree. In this study, we performed candidate gene association mapping for girth growth in rubber trees using intron length polymorphism markers (ILP) in identifying the candidate genes responsible for girth growth. The COBL064_1 marker developed from the candidate gene (COBL4) regulating cellulose deposition and oriented cell expansion in the plant cell wall showed the strongest association with girth growth across two seasons in the Amazonian population and was validated in the breeding lines. We then applied single molecule real-time (SMRT) circular consensus sequencing (CCS) to analyze a wider gene region of the COBL4 to pinpoint the single nucleotide polymorphism (SNP) that best explains the association with the traits. A SNP in the 3' UTR showing linkage disequilibrium with the COBL064_1 most associated with girth growth. This study showed that the cost-effective method of ILP gene-based markers can assist in identification of SNPs in the candidate gene associated with girth growth. The SNP markers identified in this study added useful markers for the improvement of girth growth in rubber tree breeding programs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据