期刊
PLANTS-BASEL
卷 10, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/plants10061239
关键词
maize; kernel sweetness; shrunken2; genome-wide association study (GWAS); single-nucleotide polymorphism (SNP)
资金
- National Science and Technology Development Agency (NSTDA) [P-17-52167]
Through genome-wide association study, key genes affecting sweetness trait in sweet corn were identified, and SNP markers capable of quickly distinguishing sweet corn varieties were successfully developed and validated in inbred, recombinant inbred, and popular corn varieties.
Sweetness is an economically important eating quality trait for sweet-corn breeding. To investigate the genetic control of the sweetness trait, we conducted a genome-wide association study (GWAS) in an association panel consisting of 250 sweet corn and waxy corn inbred and recombinant inbred lines (RILs), together with the genotypes obtained from the high-density 600K maize genotyping single-nucleotide polymorphism (SNP) array. GWAS results identified 12 significantly associated SNPs on chromosomes 3, 4, 5, and 7. The most associated SNP, AX_91849634, was found on chromosome 3 with a highly significant p-value of <= 1.53 x 10(-14). The candidate gene identified within the linkage disequilibrium (LD) of this marker was shrunken2 (Zm00001d044129; sh2), which encodes ADP-glucose pyrophosphorylase (AGPase), a 60 kDa subunit enzyme that affects starch metabolism in the maize endosperm. Several SNP markers specific to variants in sh2 were developed and validated. According to the validation in a set of 81 inbred, RIL, and popular corn varieties, marker Sh2_rs844805326, which was developed on the basis of the SNP at the position 154 of exon 1, was highly efficient in classifying sh2-based sweet corn from other types of corn. This functional marker is extremely useful for marker-assisted breeding in sh2-sweet corn improvement and marketable seed production.
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