Bacillus thuringiensis and Silicon Modulate Antioxidant Metabolism and Improve the Physiological Traits to Confer Salt Tolerance in Lettuce

We investigated the impact of Bacillus thuringiensis as seed treatment and application with silicon on lettuce plants exposed to salinity levels (4 dS m(-1) and 8 dS m(-1)). Results revealed that leaves number, head weight, total yield, relative water content (RWC), and chlorophyll a and b declined considerably due to two salinity levels. Oxidative stress markers, i.e., hydrogen peroxide (H2O2), superoxide (O-2(-)), and lipid peroxidation (MDA) dramatically augmented in stressed plants. On the other hand, leaves number, total yield, RWC, and chlorophyll a, b in stressed lettuce plants were considerably enhanced because of the application of Si or B. thuringiensis. In contrast, EL%, MDA, and H2O2 were considerably reduced in treated lettuce plants with Si and B. thuringiensis. In addition, the treatment with Si and B. thuringiensis increased head weight (g) and total yield (ton hectare-1), and caused up-regulation of proline and catalase, superoxide dismutase, peroxidase, and polyphenol oxidase activity in lettuce leaves under salinity conditions.

Automated summary

The study found that the application of silicon and Bacillus thuringiensis significantly reduced oxidative stress levels in lettuce plants exposed to salinity, while also enhancing leaf area, total yield, relative water content, and chlorophyll levels. Additionally, the treatment increased the expression of proline and antioxidant enzyme activity in lettuce leaves under salinity conditions.