Characterization of Chlamydomonas reinhardtii Mutants That Exhibit Strong Positive Phototaxis

The most motile phototrophic organisms exhibit photo-induced behavioral responses (photobehavior) to inhabit better light conditions for photosynthesis. The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to study photobehavior. Several years ago, we found that C. reinhardtii cells reverse their phototactic signs (i.e., positive and negative phototaxis) depending on the amount of reactive oxygen species (ROS) accumulated in the cell. However, its molecular mechanism is unclear. In this study, we isolated seven mutants showing positive phototaxis, even after the induction of negative phototaxis (ap1 similar to 7: always positive) to understand the ROS-dependent regulatory mechanism for the phototactic sign. We found no common feature in the mutants regarding their growth, high-light tolerance, and photosynthetic phenotypes. Interestingly, five of them grew faster than the wild type. These data suggest that the ROS-dependent regulation of the phototactic sign is not a single pathway and is affected by various cellular factors. Additionally, the isolation and analyses of mutants with defects in phototactic-sign regulation may provide clues for their application to the efficient cultivation of algae.

Automated summary

Researchers isolated seven mutants with positive phototaxis to study the ROS-dependent regulatory mechanism, suggesting that the regulation of phototactic sign is not a single pathway and is influenced by various cellular factors. Interestingly, five mutants showed faster growth rates than the wild type, indicating potential clues for efficient algae cultivation by analyzing mutants with defects in phototactic-sign regulation.