期刊
ENVIRONMENTAL TECHNOLOGY & INNOVATION
卷 24, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.eti.2021.101844
关键词
Starch; Consolidated bioprocessing (CBP); Alcoholic fermentation; Cell factory; Tubular cellulose
资金
- project Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation - Food Innovation RI, Greece - Operational Programme Competitiveness, Entrepreneurship and Innovation, Greece (NSRF 2014-2020) [MIS 5027222]
- European Union (European Regional Development Fund)
The utilization of cell factory (CF) for starch alcoholic fermentation shows promising results in terms of improving production efficiency and achieving higher ethanol yield, productivity, and yield. The study indicates that freeze-dried CF could be a more effective biocatalyst for starch fermentation, offering potential benefits for environmental conservation and cost-effectiveness.
The exploitation of starchy wastes for the production of value-added products and the consolidated bioprocessing (CBP) have a positive environmental and cost-effective impact. For this reason, a cell factory (CF) was employed to perform three bioprocesses in one step (CBP) for alcoholic fermentation of starch without genetic modification. CF is a bilayer biocatalyst consisting of an inner layer of immobilized Saccharomyces cerevisiae on tubular cellulose (TC) and an external layer of immobilized Aspergillus awamori on alginates (ALG). In first, the suggested CF was proved to be more effective compared with co-immobilized S. cerevisiae and A. awamori on ALG beads for starch fermentation. Subsequently, the effect of the (i) S. cerevisiae concentration (g S. cerevisiae/g TC) during its immobilization, (ii) CaCl2 concentration (% w/v), (iii) form of bilayer CF (wet or freeze-dried) and (iv) freeze-dried CF concentration on 5% (w/v) starch fermentation was examined. It was found that the higher ethanol production (32.17 mL/L), productivity (4.60 mL/L/d) and yield (0.51 g ethanol/g starch) reaching the theoretical one were obtained when the CF in freeze dried form fermented 100 mL of 5% (w/v) starch solution. Finally, two repeated fermentation batches were performed using the best CF. The ethanol yield was decreased during the repeated fermentation batches, but remained in acceptable levels. The successful preparation of CF was verified with FTIR spectroscopy, SEM and TEM analysis. The results indicated that the objective of designing CF was successfully achieved, and the results are promising to be the base for the exploitation of starchy wastes and the development of an innovation in brewing industry by eliminating the malting stage. This CF can be applied as model for different bioprocesses of White Biotechnology, e.g. substituting S. cerevisiae with the appropriate microorganism to produce other metabolites of added value. (C) 2021 Elsevier B.V. All rights reserved.
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