Anti-bacterial effect of marine sea grasses mediated endophytic actinomycetes against K. pneumoniae

In this study, the pure, pale white, powdery nature and pigment produced endophytic actinomycetes strains were isolated from marine Sea grasses sample. The isolated strains were endophytes as confirmed by surface sterilization and validation methods. The anti-bacterial efficacy materials of the crude compound were extracted by liquid-liquid extraction using various organic solvents. Anti-bacterial efficacy of the isolated actinomycetes strain RMN 10 was exhibited excellent anti-bacterial activity against K. pneumoniae at increasing concentration. In addition, the minimum inhibition concentration (MIC) of the crude actinomycetes compound has excellent anti-bacterial activity at 250 mg/mL concentration and this concentration was fixed as a MIC. Further, the structural and shape damages of actinomycetes crude compound treated K. pneumoniae cells were clearly identified in SEM images. All the invitro experiments were proved that the actinomycetes compound has excellent anti-bacterial activity against K. pneumoniae. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

Automated summary

In this study, endophytic actinomycetes strains were isolated from marine Sea grasses sample, and their crude compounds showed excellent antibacterial activity against K. pneumoniae. The structural damages of treated cells were clearly identified in SEM images, confirming the strong antibacterial efficacy of the actinomycetes compound.