期刊
BRITISH JOURNAL OF NUTRITION
卷 113, 期 1, 页码 35-44出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S000711451400347X
关键词
Apoptosis; Diabetic nephropathy; Grape seed procyanidin B2; Mesangial cells; Mitochondrial dysfunction
资金
- National Natural Science Foundation of China [81102116, 81072293]
- Research Fund for the Doctoral Program of Higher Education [20110001120026]
Grape seed procyanidin B2 (GSPB2), an antioxidative and anti-inflammatory polyphenol in grape seed, has been found to have protective effects on diabetic nephropathy. Based on its favourable biological activities, in the present study, we aimed to investigate whether GSPB2 could inhibit apoptosis in rat mesangial cells treated with glucosamine (GlcN) under high-dose conditions. The results showed that the administration of GSPB2 (10 mu g/ml) significantly increased the viability of mesangial cells treated with GlcN at a dose of 15 mm. We found that GSPB2 inhibited apoptosis in mesangial cells using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphates (dUTP) nick-end labelling staining and flow cytometry technique (P<0.05 for both). GSPB2 treatment also suppressed oxidative stress by elevating the activity of glutathione peroxidase (P<0.05) and superoxide dismutase (P<0.01), as well as prevented cellular damage. GSPB2 enhanced the mRNA expression of nuclear respiratory factor 1, mitochondrial transcription factor A and mitochondrial DNA copy number in mesangial cells as determined by real-time PCR (P<0.05 for each). Finally, GSPB2 treatment activated the protein expression of PPAR? co-activator-1 alpha (PGC-1 alpha), silent mating type information regulation 2 homologue 1 (SIRT1) and AMP-activated protein kinase (AMPK) in mesangial cells. These findings suggest that GSPB2 markedly ameliorates mitochondrial dysfunction and inhibits apoptosis in rat mesangial cells treated with high-dose GlcN. This protective effect could be, at least in part, due to the activation of the AMPKSIRT1PGC-1 alpha axis.
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