期刊
ANTIBIOTICS-BASEL
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/antibiotics10070772
关键词
methicillin-resistant staphylococci; bovine mastitis; antibiotic resistance genes; S; aureus coagulase-negative staphylococci
资金
- Consortium for Advanced Research Training in Africa (CARTA)
- Carnegie Corporation of New York [G-19-57145]
- Sida [54100113]
- Uppsala Monitoring Centre
- DELTAS Africa Initiative [107768/Z/15/Z]
- New Partnership for Africa's Development Planning and Coordinating Agency (NEPAD Agency)
- Wellcome Trust (UK)
- UK government
The study revealed a high prevalence of multidrug-resistant staphylococci strains associated with mastitis in dairy cows in Kenya, posing a threat to both animal and human health. Different resistance genes and multidrug resistance were observed in both Staphylococcus aureus and coagulase-negative staphylococci species.
Increasing numbers of potentially zoonotic multidrug-resistant (MDR) staphylococci strains, associated with mastitis in dairy cows, are being reported globally and threaten disease management in both animal and human health. However, the prevalence and antimicrobial resistance profiles of these strains, including methicillin-resistant staphylococci (MRS), in Kenya is not well known. This study investigated the drug resistance profiles and genes carried by 183 staphylococci isolates from 142 dairy cows representing 93 farms recovered from mastitis milk of dairy cows in two selected counties in Kenya. Staphylococci isolates were characterized by phenotypic characteristics, polymerase chain reaction (PCR) amplification, partial sequencing and susceptibility testing for 10 antimicrobial drugs. Detection of seven resistance genes to the various antimicrobial drugs was conducted using PCR. Overall, phenotypic resistance among the staphylococci ranged between 66.1% for ampicillin and 3.5% for fluoroquinolones. Twenty-five percent (25%) of S. aureus and 10.8% of the coagulase-negative staphylococci (CoNS) isolates, were methicillin-resistant staphylococci phenotypically (defined as resistance to cefoxitin disk diffusion). The most common genes found in S. aureus and CoNS were blaZ and strB at 44.3% and 26%, and 78% and 50%, respectively. MDR was observed in 29.67% and 16.3% of S. aureus and CoNS, respectively. These findings pose a threat to bovine mastitis treatment and management as well as human health.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据