期刊
FOODS
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/foods10092164
关键词
Porphyridium purpureum; fractional extraction; phycoerythrin; polyunsaturated fatty acid; polysaccharides
资金
- Key-Area Research and Development Program of Guangdong Province [2020B1111030004]
- Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0406]
- Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development [E039kf0301]
- Natural Science Foundation for research team of Guangdong Province [2016A030312007]
A novel fractional extraction strategy was developed to efficiently extract high-value compounds from Porphyridium, including phycoerythrin, lipids, and polysaccharides. Although crude phycoerythrin showed the best antioxidant activity, further purification is required for downstream applications.
The microalga Porphyridium accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of Porphyridium biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.3, 74.3, and 75.2%, respectively. The phycoerythrin exhibited excellent fluorescence characteristics but had low purity. The crude lipid was dark with poor fluidity. Digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol containing C20:5 and C20:4 were the most abundant glycerolipids, while glucose, xylose, and galactose constituted the intracellular polysaccharides that had covalently bound to proteins (8.01%), uronic acid (4.13%), and sulfate (8.31%). Compared with polysaccharides and crude lipids, crude phycoerythrin showed the best antioxidant activity. Overall, the three-step fractional extraction process was feasible for Porphyridium; however, further purification is necessary for downstream applications.
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