期刊
FOODS
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/foods10081838
关键词
time-resolved fluorescence immunochromatographic assay; tylosin; tilmicosin; milk
资金
- National Key Research and Development of China [2018YFC1602904]
- Guangdong Basic and Applied Basic Research Foundation [2019B1515210025, 2020A1515011238, 2018B030314005]
- Science and Technology Planning Project of the Guangxi [AB18126048]
- Science and Technology Planning Project of Guangzhou City [202102080615]
- Key-Area Research and Development Program of Guangdong Province [2019B02021101]
A quantitative and visual TRFICA method was developed for detecting T&T residues in milk, with low detection limits, short detection time, and good stability.
Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87 similar to 7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 degrees C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.
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