期刊
FOODS
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/foods10091973
关键词
hepatoprotective peptide; AGL9; hepatic lipid metabolism; inflammation; nonalcoholic fatty liver disease
资金
- Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration, Korea [PJ016169]
- Ministry of Agriculture, Food, and Rural Affairs [MAFRA 316027-5]
- Ministry of Education, Science, and Technology through the National Research Foundation of Korea (NRF) [NRF-2020R1A2C1014798]
The novel peptide AGL9 extracted from Allomyrina dichotoma larvae demonstrated anti-obesity properties by normalizing serum levels and regulating gene expression in liver cells. This study suggests that AGL9 may serve as a potential therapeutic strategy for nonalcoholic fatty liver disease (NAFLD) by attenuating lipid deposition, oxidative stress, and inflammation through AMPK/Nrf2 signaling inhibition.
In this study, we investigated the anti-obesity properties of the novel peptide Ala-Gly-Leu-Gln-Phe-Pro-Val-Gly-Arg (AGL9), isolated from the enzymatic hydrolysate of Allomyrina dichotoma larvae. To investigate the preventive effects of AGL9 against hepatic steatosis and its possible mechanisms of action, we established an nonalcoholic fatty liver disease (NAFLD) model by feeding C57BL/6 mice a high-fat diet. NAFLD mice were administered 100 mg/kg AGL9 and 60 mg/kg orlistat via gavage (10 mL/kg) for 5 weeks, followed by the collection of blood and liver tissues. We found that AGL9 normalized the levels of serum alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol, high-density lipoprotein, very low-density lipoprotein (LDL)/LDL, adiponectin, and leptin in these mice. Additionally, AGL9 activated the protein-level expression of 5 ' AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation and the transcript-level expression of sterol regulatory element-binding protein-1c, fatty acid synthase, superoxide dismutase, glutathione peroxidase, glucocorticoid receptor, nuclear respiratory factor 2, tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and monocyte chemoattractant protein-1 in hepatocytes. These results showed that AGL9 exhibited hepatoprotective effects by attenuating lipid deposition, oxidative stress, and inflammation via inhibition of AMPK/Nrf2 signaling, thereby reducing the production of hepatic proinflammatory mediators and indicating AGL9 as a potential therapeutic strategy for NAFLD.
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