期刊
REGENERATIVE BIOMATERIALS
卷 8, 期 4, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/rb/rbab030
关键词
type I collagen; contraction; photo-crosslinkable hydrogel; chondrocyte
资金
- National Key Research and Development Program of China [2019YFA0110600]
- Science and Technology Support Program of Sichuan Province [2019YJ0161]
- Guangxi Key Research and Development Plan [GuikeAB16450003]
The study modified type I collagen with photo-crosslinkable methacrylate anhydride and found that adjusting its physical properties by varying the degree of substitution could improve storage modulus and degradation rate of hydrogels. Results demonstrated that CM hydrogels with suitable DS could resist contraction, promote chondrocyte behaviors, and enhance secretion of cartilage-specific matrix in vitro and in vivo.
Collagen is a promising material for tissue engineering, but the poor mechanical properties of collagen hydrogels, which tend to cause contraction under the action of cellular activity, make its application challengeable. In this study, the amino group of type I collagen (Col I) was modified with methacrylic anhydride (MA) and the photo-crosslinkable methacrylate anhydride modified type I collagen (CM) with three different degrees of substitution (DS) was prepared. The physical properties of CM and Col I hydrogels were tested, including micromorphology, mechanical properties and degradation properties. The results showed that the storage modulus and degradation rate of hydrogels could be adjusted by changing the DS of CM. In vitro, chondrocytes were seeded into these four groups of hydrogels and subjected to fluorescein diacetate/propidium iodide (FDA/PI) staining, cell counting kit-8 (CCK-8) test, histological staining and cartilage-related gene expression analysis. In vivo, these hydrogels encapsulating chondrocytes were implanted subcutaneously into nude mice, then histological staining and sulfated glycosaminoglycan (sGAG)/DNA assays were performed. The results demonstrated that contraction of hydrogels affected behaviors of chondrocytes, and CM hydrogels with suitable DS could resist contraction of hydrogels and promote the secretion of cartilage-specific matrix in vitro and in vivo.
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