期刊
PATHOGENS
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/pathogens10081008
关键词
wastewater-based epidemiology; SARS-CoV-2; COVID-19; coronavirus; detection and quantification protocols
类别
资金
- Research Vice-Chancellor of Qazvin University of Medical Sciences [IR.QUMS.REC.1399.543]
- Iran's National Elites Foundation (INEF) [15/11529]
- Agencia Nacional de Investigacion y Desarrollo (ANID-FONDECYT, Chile) [3200274]
Wastewater-based epidemiology (WBE) has a long history of identifying various viruses, including SARS-CoV-2. Studies have shown a correlation between the presence of SARS-CoV-2 RNA in wastewater and the number of new positive cases, suggesting WBE as a faster protocol to alert public health authorities to take actions during pandemics.
Wastewater-based epidemiology (WBE) has a long history of identifying a variety of viruses from poliovirus to coronaviruses, including novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The presence and detection of SARS-CoV-2 in human feces and its passage into the water bodies are significant public health challenges. Hence, the hot issue of WBE of SARS-CoV-2 in the coronavirus respiratory disease (COVID-19) pandemic is a matter of utmost importance (e.g., SARS-CoV-1). The present review discusses the background, state of the art, actual status, and prospects of WBE, as well as the detection and quantification protocols of SARS-CoV-2 in wastewater. The SARS-CoV-2 detection studies have been performed in different water matrixes such as influent and effluent of wastewater treatment plants, suburban pumping stations, hospital wastewater, and sewer networks around the globe except for Antarctica. The findings revealed that all WBE studies were in accordance with clinical and epidemiological data, which correlates the presence of SARS-CoV-2 ribonucleic acid (RNA) with the number of new daily positive cases officially reported. This last was confirmed via Reverse Transcriptase-quantitative Polymerase Chain Reaction (RT-qPCR) testing which unfortunately is not suitable for real-time surveillance. In addition, WBE concept may act as a faster protocol to alert the public health authorities to take administrative orders (possible re-emerging infections) due to the impracticality of testing all citizens in a short time with limited diagnostic facilities. A comprehensive and integrated review covering all steps starting from sampling to molecular detection of SARS-CoV-2 in wastewater has been made to guide for the development well-defined and reliable protocols.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据