期刊
PATHOGENS
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/pathogens10070861
关键词
coronavirus peptide; diamond nanoparticles; neutrophil extracellular traps
类别
资金
- Centre National de la Recherche Scientifique (CNRS)
- University of Lille
- ANR
- CPER Photonics for Society
- Agence Nationale de la Recherche (ANR-nanoMERS)
- Ministry of Healthcare of Ukraine [0119U101338]
- National Research Foundation of Ukraine [2020.02/0131]
- Volkswagen-Stiftung [97744]
- European Union's Horizon 2020 research and innovation program [861878]
This study introduces a method of antigen conjugation using nanodiamond-based adjuvants to induce immune response through neutrophil-driven self-limiting inflammation. Experimental results demonstrate that the adjuvant is safe and effective, capable of inducing persistent high-level antibodies in animal models.
Vaccination remains one of the most effective tools to prevent infectious diseases. To ensure that the best possible antigenic components are chosen to stimulate a cognitive immune response, boosting antigen presentation using adjuvants is common practice. Nanodiamond-based adjuvants are proposed here as a rapid and versatile platform for antigen conjugation, utilizing peptides common to different pathogenic strains and making this strategy a good candidate for a ready-to-use vaccine. Initiation of an inflammatory reaction with a resulting immune response is based on the ability of living organisms to entrap nanostructures such as nanodiamonds with neutrophil extracellular traps (NETs) formation. In this work, coronavirus peptide homological for MERS-CoV, fusion inhibitor, was conjugated to nanodiamonds and used to induce neutrophilic-driven self-limiting inflammation. The resulting adjuvant was safe and did not induce any tissue damage at the site of injection. Mice immunization resulted in IgG titers of 1/4,000 within 28 days. Immunization of rabbits resulted in the formation of a high level of antibodies persistently present for up to 120 days after the first immunization (animal lifespan similar to 3 years). The peptide used for immunization proved to be reactive with sera of convalescent COVID patients, demonstrating the possibility of developing pancoronaviral vaccine candidates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据