期刊
PATHOGENS
卷 10, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/pathogens10070903
关键词
take-all; biocontrol; growth-promoting; Pseudomonas chlororaphis; Gaeumannomyces graminis var; tritici
类别
资金
- Science-Technology Foundation for Outstanding Young Scientists of Henan Academy of Agricultural Sciences [2018YQ16]
- Major Achievement Cultivation Program of Henan Academy of Agricultural Sciences [20191101004]
- Independent Innovation Special Fund of Henan Academy of Agricultural Sciences [2020ZC31, 2020ZC56]
- Central Government Guiding Local Projects [2020 [44]]
Pseudomonas chlororaphis YB-10 effectively inhibits the growth of Gaeumannomyces graminis var. tritici and controls take-all disease when coated on wheat seeds, promoting seedling growth. Additionally, the bacterium enhances wheat seedlings' defense enzyme activities, inducing resistance against pathogens.
Wheat is a worldwide staple food crop, and take-all caused by Gaeumannomyces graminis var. tritici can lead to a tremendous decrease in wheat yield and quality. In this study, strain YB-10 was isolated from wheat rhizospheric soil and identified as Pseudomonas chlororaphis by morphology and 16S rRNA gene sequencing. Pseudomonas chlororaphis YB-10 had extracellular protease and cellulase activities and strongly inhibited the mycelium growth of Gaeumannomyces graminis var. tritici in dual cultures. Up to 87% efficacy of Pseudomonas chlororaphis YB-10 in controlling the take-all of seedlings was observed in pot experiments when wheat seed was coated with the bacterium. Pseudomonas chlororaphis YB-10 was also positive for indole acetic acid (IAA) and siderophore production, and coating wheat seed with the bacterium significantly promoted the growth of seedlings at 10(7) and 10(8) CFU/mL. Furthermore, treatment with Pseudomonas chlororaphis YB-10 increased activities of the wheat defense-related enzymes POD, SOD, CAT, PAL and PPO in seedlings, indicating induced resistance against pathogens. Overall, Pseudomonas chlororaphis YB-10 is a promising new seed-coating agent to both promote wheat growth and suppress take-all.
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