期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2021.714238
关键词
xylobiohydrolase; glycoside hydrolase; glycoside hydrolase family 30; AcXbh30A; Acetivibrio clariflavus; xylobiose; glucuronoxylan; Hungateiclostridium clariflavum
资金
- Institute for Microbial and Biochemical Technology (IMBT) of the Forest Products Laboratory (FPL), a National Laboratory of the USDA Forest Service
- U.S. Department of Energy, Office of Biological and Environmental Research [DE-AC02-06CH11357]
This study characterized a non-reducing terminal (NRT)-specific xylobiohydrolase AcXbh30A, which strictly cleaves xylobiose from the NRT with minimal other hydrolysis products under optimized reaction conditions. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.
The Acetivibrio clariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an alpha-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.
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