4.6 Review

Electronic Couplings and Electrostatic Interactions Behind the Light Absorption of Retinal Proteins

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Summary: This study introduces simple models to estimate the direct effect of charged or polar residue substitutions in proteins from the rhodopsin family, without the need for extensive calculations. The models are based on absorption maximum values calculated at a theoretical level for the retinal chromophore in an external electrostatic field. The proposed approach was evaluated against various mutants and can be useful for interpreting experimental data and designing proteins with desired spectral properties.

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