期刊
MICROORGANISMS
卷 9, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9061299
关键词
Crohn's disease; Escherichia coli; whole-genome sequencing; shotgun metagenome sequencing
类别
资金
- Kazan Federal University [0671-2020-0058]
- Russian Foundation for Basic Research [17-00-00433]
Crohn's disease is characterized by chronic inflammation in the gastrointestinal tract, where an imbalance in gut microbiota, particularly Escherichia coli, plays a significant role in its pathogenesis. Analysis of E. coli strains from CD patients and healthy subjects revealed genetic similarities in virulence and antibiotic resistance, suggesting complex bacterial interactions within the gut that may impact the host's intestinal health.
Crohn's disease (CD) is characterized by a chronic, progressive inflammation across the gastrointestinal tract with a series of exacerbations and remissions. A significant factor in the CD pathogenesis is an imbalance in gut microbiota composition, particularly the prevalence of Escherichia coli. In the present study, the genomes of sixty-three E. coli strains from the gut of patients with CD and healthy subjects were sequenced. In addition, eighteen E. coli-like metagenome-assembled genomes (MAGs) were reconstructed from the shotgun-metagenome sequencing data of fecal samples. The comparative analysis revealed the similarity of E. coli genomes regardless of the origin of the strain. The strains exhibited similar genetic patterns of virulence, antibiotic resistance, and bacteriocin-producing systems. The study showed antagonistic activity of E. coli strains and the metabolic features needed for their successful competition in the human gut environment. These observations suggest complex bacterial interactions within the gut which may affect the host and cause intestinal damage.
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